ABSTRACT
RESUMO O Tumor Venéreo Transmissível Canino (TVTC) é uma neoplasia de células redondas que tem a particularidade de se implantar em mucosas que tenham perdido a sua integridade. Nesse local o tumor prolifera e ocasionalmente origina metástase. Em geral, o tumor responde ao tratamento com sulfato de vincristina, porém a resistência quimioterápica associada ao fenótipo tumoral tem sido documentada. Objetivou-se relatar um caso de TVTC genital de fenótipo citológico misto com metástase esplênica e o insucesso da quimioterapia com sulfato de vincristina, em uma fêmea canina, da raça Australian Cattle Dog, de cinco anos de idade. Após diagnóstico citológico e histológico, o tumor primário foi ainda caracterizado em fase de progressão e mostrou baixa expressão de moléculas do complexo principal de histocompatibilidade (MHC) (4,4 ± 2% classe I e 11 ± 4,1% classe II). A cadela foi submetida à ovariohisterectomia e esplenectomia terapêutica e não apresentou recidiva do tumor após 12 meses de acompanhamento clínico.
ABSTRACT The canine transmissible venereal tumor is a type of round cell cancer that have the particularity of implanting in mucous tissue, when they lose their integrity, at which point the tumour proliferates and may even develop metastases. The tumor typically responds well to vincristine sulfate chemotherapy, although there are cases of resistance to the drug correlated with the tumoral phenotype. We describe herein a genital mixed TVTC case with metastases at spleen and failure at vincristine sulfate chemotherapeutic treatment in a five years old Australian Cattle Dog female. After the cytological, histological and cytogenetic diagnostic, the primary tumor was still characterized in progression phase and showed low major histocompatibility complex expression MHC (4,4 ± 2% class I e 11 ± 4,1% class II. The dog underwent therapeutic splenectomy and ovariohysterectomy and did not present tumor recurrence within 12 months of clinical follow-up.
Subject(s)
Animals , Venereal Tumors, Veterinary , Vincristine , Dogs , Genitalia , Hysterectomy , Mucous Membrane , Neoplasm Metastasis , Neoplasms , Recurrence , Splenectomy , Sulfates , Therapeutics , Tissues , Pharmaceutical Preparations , Drug Therapy , HistocompatibilityABSTRACT
O objetivo deste estudo foi avaliar a influência do plasma rico (PRP) e pobre (PPP) em plaquetas na proliferação celular e expressão de metaloproteinases de matriz (MMPs), durante a reparação de úlceras corneais profundas. Foram utilizadas 45 coelhas, distribuídas em 3 grupos (G) experimentais (n=15), designados como grupos PRP (GR), PPP (GP) e Controle (GC), de acordo com o tratamento. Todos os animais foram submetidos à indução cirúrgica unilateral de úlcera corneal. No GR e GP, o sangue autólogo foi centrifugado, utilizando-se protocolo padronizado, e foram confeccionados os colírios de PRP e PPP, e instilados cinco vezes ao dia. No GC, foi utilizado colírio lubrificante. Cada grupo foi subdividido (n=5), segundo o momento final de avaliação, sendo 4 (M4), 7 (M7) e 30 dias (M30). As córneas dos animais foram processadas para avaliação morfológica e imuno-histoquímica para PCNA, MMP1, MMP2, MMP9, MT1-MMP e TIMP1. No M4, os níveis de MMP2 foram maiores no GP e GR, sendo que, no M7, esse comportamento foi observado apenas no GP. No M30, no GR, verificou-se maior número de células epiteliais e marcação para MMP1 que o GP. No GR, a proliferação celular foi maior no M4 que nos demais momentos, e a marcação para MMP2 foi maior no M4 que no M30. O PRP estimula a proliferação celular na fase inicial (M4) do tratamento quando comparado aos demais momentos, diferentemente dos demais tratamentos. O uso de colírios de plasma rico e pobre em plaquetas influencia a expressão de metaloproteinases de matriz envolvidas no processo de reparação corneal.
The aim of this study was to evaluate the influence of platelet-rich (PRP) and poor (PPP) plasma in cell proliferation and matrix metalloproteinases (MMPs) expression during the repair of deep corneal ulcers. Forty-five female rabbits were distributed in 3 experimental groups (G) (n = 15), referred to as PRP (GR), PPP (GP) and Control (GC) groups, in accordance with the treatment. All animals underwent surgical induction of unilateral corneal ulcer. PRP and PPP eye drops were made by using centrifuged blood through standardized protocol, and instilled five times a day. In GC, lubricant eye drops were used. Each group was subdivided (n = 5) according to the final time point, 4 (M4), 7 (M7) and 30 days (M30). The animals' corneas were processed for morphological and immunohistochemical analysis for PCNA, MMP1, MMP2, MMP9, MT1-MMP and TIMP1. In M4, the levels of MMP2 were higher in GP and GR, and in M7, this behavior was only observed in the GP. In M30, more epithelial cells and MMP1 expression were found in GR than GP. In GR, cell proliferation was higher in M4 than at other time points and MMP2 expression was higher in M4 than M30. The PRP stimulates cell proliferation in the early phase (M4) of treatment when compared to other time points, different from other treatments. The use of eye drops of platelet-rich and poor plasma influences the expression of matrix metalloproteinases involved in the corneal repair process.
Subject(s)
Animals , Female , Rabbits , Proliferating Cell Nuclear Antigen/analysis , Matrix Metalloproteinases/analysis , Platelet-Rich Plasma/physiology , Corneal Ulcer/surgery , Wound Healing/physiology , Immunohistochemistry/veterinary , Corneal Injuries/veterinary , Cell Proliferation/physiologyABSTRACT
A Criptococose é uma importante doença infecciosa fúngica, causada por uma levedura do gênero Cryptococcus, que acomete diferentes espécies inclusive o homem. Há poucos relatos na literatura sobre a criptococose pulmonar em cães. O presente trabalho relata um caso de criptococose em um cão apresentando alterações respiratórias, especialmente dispneia. O diagnóstico foi realizado por meio da citologia aspirativa, após toracotomia exploratória, sendo observado um quadro de pleuris grave. O paciente foi tratado durante 90 dias com itraconazol e apresentou, ao final do tratamento, a remissão completa dos sintomas relatados...
Cryptococcosis is a major infectious disease caused by a yeast from the Cryptococcus genre which affects different species including humans. There are few reports related to pulmonary cryptococcosis in dogs. A case of Cryptococcosis in a dog showing dyspnea was described. The diagnosis was done by aspiration cytology after exploratory thoracotomy, and a picture of severe pleurisy was observed. The diagnosis was done by needle aspiration cytology after thoracotomy. Severe pleuris frame was observed. Treatment with itraconazole was performed for 90 days, and at the end of the treatment the dog presented complete remission of reported symptoms...
Subject(s)
Animals , Dogs , Cryptococcosis/veterinary , Itraconazole/therapeutic use , Pleurisy/diagnosis , Pleurisy/veterinary , Cell Biology , Dyspnea/veterinary , Thoracotomy/veterinaryABSTRACT
Dogs and cats are the animals that owners most frequently seek assistance for potential poisonings, and these species are frequently involved with toxicoses due to ingestion of poisonous food. Feeding human foodstuff to pets may prove itself dangerous for their health, similarly to what is observed in Allium species toxicosis. Allium species toxicosis is reported worldwide in several animal species, and the toxic principles present in them causes the transformation of hemoglobin into methemoglobin, consequently resulting in hemolytic anemia with Heinz body formation. The aim of this review is to analyze the clinicopathologic aspects and therapeutic approach of this serious toxicosis of dogs and cats in order to give knowledge to veterinarians about Allium species toxicosis, and subsequently allow them to correctly diagnose this disease when facing it; and to educate pet owners to not feed their animals with Allium-containg food in order to better control this particular life-threatening toxicosis.
Subject(s)
Animals , Cats , Allium/toxicity , Anemia, Hemolytic/drug therapy , Cats , Dogs , Plant Preparations/pharmacology , Heinz BodiesABSTRACT
Uninfected dogs (n = 10) and those naturally infected with leishmaniasis (n = 10) were subjected to several diagnostic tests, namely: hemoculture, polymerase chain reaction (PCR) of hemoculture, indirect immunofluorescence (RIFI), cytological examination of lymph node aspirate, culture of lymph node aspirate and PCR of lymph node aspirate. RIFI - followed by PCR of lymph node aspirate culture - presented more positive results in infected dogs than in uninfected ones. In infected animals, RIFI was more effective than PCR of lymph node aspirate culture. There was no statistical difference in positivity between RIFI and hemoculture; lymph node aspirate culture/cytological examination of lymph node aspirate and PCR of hemoculture; and between PCR of lymph node aspirate culture and PCR of hemoculture. All infected and uninfected animals had positive and negative results in at least one test. In conclusion, the association of several tests improves the efficacy of canine visceral leishmaniasis diagnosis.
Subject(s)
Animals , Dogs , Leishmaniasis, Visceral/diagnosisABSTRACT
Foram utilizados anticorpos monoclonais para marcaçäo imunoistoquímica dos tecidos tumorais e obtençäo de informaçöes sobre a histogênese dos tumores mamários utilizando-se anti-citoqueratinas para marcaçäo de células epiteliais, e anti-actina e anti-vimentina para células mioepiteliais. O procedimento imunoistoquímico mostrou-se esclarecedor com relaçäo à histogênese dos tumores mamários, confirmando a marcaçäo de células epiteliais com as citoqueratinas que perdem sua expressäo na transformaçäo celular maligna. A alfa-actina e a vimentina mostraram-se eficientes na marcaçäo de células mioepiteliais. A alfa-actina diminuiu a marcaçäo na metaplasia óssea ou cartilaginosa contrariamente à vimentina cuja marcaçäo foi aumentada. Os resultados permitem melhor entendimento da classificaçäo dos tumores mamários de cadelas com a utilizaçäo de anticorpos monoclonais como marcadores do citoesqueleto, que se mostraram eficientes nessa caracterizaçäo
Subject(s)
Animals , Female , Breast Neoplasms , Dogs , ImmunohistochemistryABSTRACT
Relata-se a ocorrência de dois casos de pseudotumor inflamatório na bexiga de cäes, enfatizando a importância de reconhecer o caráter benigno dessas lesöes, evitando uma abordagem terapêutica agressiva como conseqüência de um diagnóstico equivocado
Subject(s)
Animals , Male , Female , Dogs , Urinary BladderABSTRACT
Fibrin glue has been used on its own or in conjunction with suturing materials to promote hemostasis, reduce adherence, strengthen the wound site, and improve healing. Snake venom derived fibrin glue was evaluated as an alternative to conventional uterine suturing after ovine caesarean surgey. Twenty-eight pregnant ewes of known mating date were used. The animals submitted to conventional caesaream sections showed a better wound healing process. As expected, all the operated animals had retained placenta, compromissing coaptation of wound edges. This had a strong influence in the results observed with the animals in which fibrin glue was used. The animals were divided into four groups GI, GII, GIII, and GIV and sacrificed, respectively, 3, 7, 15, and 30 days after surgery for macro and microscopic examination of the uterus. From each group, six animals underwent surgery using fibrin glue and four animals were submitted to comparative conventional hysterorrhaphy using catgut.
Subject(s)
Animals , Female , Pregnancy , Cesarean Section , Wound Healing , Fibrin Tissue Adhesive/pharmacology , Hysterectomy , Tissue Adhesives/pharmacology , Sheep , Suture Techniques/trendsABSTRACT
Estudaram-se as características anatomoclínicas dos linfomas em cäes da regiäo de Botucatu, Säo Paulo. O material utilizado foi colhido de 34 cäes portadores de linfoma maligno, dos quais nove eram da raça Pastor-Alemäo, nove sem raça definida, cinco da raça Boxer, três animais da raça Dobermann e oito outros cada um de uma raça, 68 por cento deles machos. A idade variou de 1 a 13 anos, com média de 6,2 anos. O estabelecimento do estádio dos linfomas foi baseado nos critérios estabelecidos pela Organizaçäo Mundial da Saúde. No momento do diagnóstico, 32 animais apresentavam-se nos estádios clínicos III, IV ou V. Apenas dois foram enquadrados no estádio II. A forma anatômica mais freqüente foi a multicêntrica, diagnosticada em 31 animais. A forma tímica, diagnosticada em dois animais, e a disgestiva, em um animal, foram as outras anatômicas encontradas
Subject(s)
Animals , Dogs , Lymphoma , NeoplasmsABSTRACT
It was described for the first time in Brazil the involvement of Prototheca alga in bovine mastitis. Treatment with propolis in dimetilsulfoxide (DMSO) resulted in clinical and microbiological cure of 84,8 per cent of treated teats